Journal: Pathogens

Article Title: Tumor Cell Plasticity in Equine Papillomavirus-Positive Versus-Negative Squamous Cell Carcinoma of the Head and Neck

doi: 10.3390/pathogens11020266

Figure Lengend Snippet: Compiled β-catenin, vimentin, and keratin single-labeling results.

Article Snippet: Mouse , Monoclonal , 9G2 , Beta-catenin , Acris Antibodies, Herford, Germany , 1:500 , pH 9.

Techniques:

Journal: Pathogens

Article Title: Tumor Cell Plasticity in Equine Papillomavirus-Positive Versus-Negative Squamous Cell Carcinoma of the Head and Neck

doi: 10.3390/pathogens11020266

Figure Lengend Snippet: β-catenin labeling of EcPV2-positive and EcPV2-negative HNSCC sections. The figure depicts a representative selection of β-catenin labeling results. ( a , b ): patchy, strong membranous β-catenin labeling of >50% of tumor cells in an EcPV2-negative oral SCC (KRA). ( a ) Bar = 150 µm, ( b ) bar = 80 µm. ( c , d ): Strong, diffuse β-catenin labeling in an EcPV2-positive, pharyngeal SCC (SAM). ( c ) Bar = 150 µm, ( d ) bar = 80 µm. ( e , f ): Centrally focused β-catenin labeling pattern in an EcPV-positive, maxillary sinusoidal SCC (HJA). ( e ) Bar = 150 µm, ( f ) bar = 80 µm. ( g , h ): Positive and negative control, i.e., β-catenin labeled ( g ) and mock labeled (( h ); no primary Ab) equine skin sections. Bars = 100 µm. Framed areas are presented at higher magnification.

Article Snippet: Mouse , Monoclonal , 9G2 , Beta-catenin , Acris Antibodies, Herford, Germany , 1:500 , pH 9.

Techniques: Labeling, Selection, Negative Control

Journal: Journal of clinical hypertension (Greenwich, Conn.)

Article Title: Juxtaglomerular cell tumor: Clinical and immunohistochemical features.

doi: 10.1111/jch.12997

Figure Lengend Snippet: FIGURE 2 Immunohistochemical findings of juxtaglomerular cell tumor. Tumor cells are diffusely positive for CD34, vimentin, and vascular endothelial growth factor (VEGF). CD117 and β-catenin immunoreactivity are present in scattered cells and epithelium, respectively, in some cases

Article Snippet: Antigen Vendor Dilution Antigen Retrieval Incubation Time p16INK4a Ventana/Roche RTU 100°C, 30 min 16 min p53 Maixin RTU Pressure cooker 60 min p57kip2 Cell Marque 1:120 Pressure cooker 60 min Ki- 67 Origene 1:500 Pressure cooker 60 min β- Catenin Origene 1:300 Pressure cooker 60 min CD34 DAKO 1:50 Pressure cooker 100 min CD117 DAKO 1:400 Pressure cooker 100 min Vimentin DAKO 1:100 Pressure cooker 100 min Cyclin D1 DAKO RTU Pressure cooker 32 min VEGF XIYA RTU Pressure cooker 40 min HER2 Ventana/Roche RTU 100°C, 30 min 32 min BRAF Ventana/Roche RTU 100°C, 30 min 16 min TFE3 ZSGB- BIO RTU Pressure cooker 90 min Abbreviations: RTU, ready to use; VEGF, vascular endothelial growth factor.

Techniques: Immunohistochemical staining

Journal: Biomolecules

Article Title: Cellular and Molecular Mechanisms of Pathogenesis Underlying Inherited Retinal Dystrophies

doi: 10.3390/biom13020271

Figure Lengend Snippet: Immunohistochemical localization of many common inherited retinal dystrophy (IRD)-associated proteins. ( A , B ) Outer segment (POS) localization of phototransduction proteins CNGB1 ( A , red), OPN1MW ( B , red), GUCY2E ( B , green), and RHO ( A , B , blue), as well as phagocytosis protein MERTK ( A , green). ( C ) Connecting cilium (>) localization of proteins RPGR (red), CEP290 (green), and acetylated TUBA1, a marker of the axoneme (blue). ( D ) Outer limiting membrane (*) localization of proteins CRB1 (red), CTNNB1 (β-catenin, green), a marker of cadherin junctions, and GS (glutamine synthetase, blue), a marker of Müller glia. ( E ) Photoreceptor localization of ABCA4 (red) and LRAT (blue), and retinal pigment epithelial (RPE) localization of RPE65 (green) and LRAT (blue). Nuclei labeled with DAPI (grey). RPE, retinal pigment epithelium; POS/IS, photoreceptor outer segments/inner segments; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars = 20 μm ( A , D ) and 10 μm ( B , C , E ).

Article Snippet: CTNNB1 , Mouse IgG 2b , 1:200 , Origene.

Techniques: Immunohistochemical staining, Marker, Labeling

Journal: Biomolecules

Article Title: Cellular and Molecular Mechanisms of Pathogenesis Underlying Inherited Retinal Dystrophies

doi: 10.3390/biom13020271

Figure Lengend Snippet: Antibodies used for Western blots and fIHC labeling of retinal sections.

Article Snippet: CTNNB1 , Mouse IgG 2b , 1:200 , Origene.

Techniques: Western Blot, Labeling